Part:BBa_K1682010
conjugation(RP4) plasmid with MHT (Methyl halide transferase) and GFP
This plasmid contains three main components: the RP4, GFP, and MHT. The RP4 gene, when transformed specifically into S17 E. coli strain, facilitates the conjugation of E.coli with most other bacteria. The plasmid also contains MHT, short for methyl halide transferase, which if provided with either Br, Cl, or I, produces its corresponding methyl halide gas. This part also contains a GFP, specifically BBa_E0040. This plasmid can be used to faciliate transformation of bacteria that are not easily transformable through heat shock or electroporation. In addition, the plasmid also have two reporter genes, which produce the GFP and the methyl halide gas.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1780
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2157
Illegal NgoMIV site found at 2283
Illegal NgoMIV site found at 2293
Illegal AgeI site found at 1592 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1814
Illegal SapI site found at 2185
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